diff --git a/config/feature_flags/development/global_file_size_check.yml b/config/feature_flags/development/global_file_size_check.yml
deleted file mode 100644
index 67651c7e091d071bc6136011ef79ccad71194836..0000000000000000000000000000000000000000
--- a/config/feature_flags/development/global_file_size_check.yml
+++ /dev/null
@@ -1,8 +0,0 @@
----
-name: global_file_size_check
-introduced_by_url: https://gitlab.com/gitlab-org/gitlab/-/merge_requests/125956
-rollout_issue_url: https://gitlab.com/gitlab-org/gitlab/-/issues/418995
-milestone: '16.2'
-type: development
-group: group::source code
-default_enabled: false
diff --git a/lib/gitlab/checks/global_file_size_check.rb b/lib/gitlab/checks/global_file_size_check.rb
index ece9e586b753196e4d9239ab47208c9fce81952a..2acc7e371e5df5fdcc379b5c33aecdb0ea1266e8 100644
--- a/lib/gitlab/checks/global_file_size_check.rb
+++ b/lib/gitlab/checks/global_file_size_check.rb
@@ -8,8 +8,6 @@ class GlobalFileSizeCheck < BaseBulkChecker
       LOG_MESSAGE = 'Checking for blobs over the file size limit'
 
       def validate!
-        return unless Feature.enabled?(:global_file_size_check, project)
-
         Gitlab::AppJsonLogger.info(LOG_MESSAGE)
         logger.log_timed(LOG_MESSAGE) do
           oversized_blobs = Gitlab::Checks::FileSizeCheck::HookEnvironmentAwareAnyOversizedBlobs.new(
diff --git a/spec/lib/gitlab/checks/global_file_size_check_spec.rb b/spec/lib/gitlab/checks/global_file_size_check_spec.rb
index b711ea15d2ac0fa32cafedbfcd4ebc9b59364a45..7efb8e0cadb5a498bb326871e852554b86904ffb 100644
--- a/spec/lib/gitlab/checks/global_file_size_check_spec.rb
+++ b/spec/lib/gitlab/checks/global_file_size_check_spec.rb
@@ -6,19 +6,6 @@
   include_context 'changes access checks context'
 
   describe '#validate!' do
-    context 'when global_file_size_check is disabled' do
-      before do
-        stub_feature_flags(global_file_size_check: false)
-      end
-
-      it 'does not log' do
-        expect(subject).not_to receive(:log_timed)
-        expect(Gitlab::AppJsonLogger).not_to receive(:info)
-        expect(Gitlab::Checks::FileSizeCheck::HookEnvironmentAwareAnyOversizedBlobs).not_to receive(:new)
-        subject.validate!
-      end
-    end
-
     it 'checks for file sizes' do
       expect_next_instance_of(Gitlab::Checks::FileSizeCheck::HookEnvironmentAwareAnyOversizedBlobs,
         project: project,